Regulatory
MiniToe-4

Part:BBa_K2615024:Design

Designed by: Anyi Li   Group: iGEM18_OUC-China   (2018-09-07)


MiniToe-4, a member of miniToe family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Csy4 binding site is a mutant of the wild type. The sequence of 22nt hairpin is changed from GTTCACTGCCGTATAGGCAGCT to GTTCAATGCCGTATAGGCATCT.

Source

References